Here´s a "how to"description of my method for keeping calanoid stocks indefinitely.It is very simple and demanding very little work or materials.
It is in fact so easy that I see no excuse why cultures of many different calanoid species are not being kept and starter cultures available for interested parties,which I´m sure are many.
If we keep in the "recipe"style,here are the ingredients:
Plastic gallon jars,wide mouthed and made of clear PET.I don´t know if they are found anywhere.They are very practical,I also use them for rearing larval shrimp (future thread).
53,100 and 200 micron sieves.I make mine out of clear sections of acrylic pipe,8x6cm.This size fits in Petri dishes and under a diss.microscope,which comes very handy for observations.
works fine and is very easy to grow.Same for TET
is said to be better,but a little more touchy.Since this is the most limiting factor,I tried to find if they can be reared with frozen TET
paste.It can be done but is very difficult,I failed 9 of every 10 times.
.I use IO
Fill a jar with one of the collected bottles and fill up to 3 L
of the same salinity.For estuarine species,such as A.tonsa,I use 1.010.
Provide gentle air bubbling.
Feed 100 ml of a dense algal culture every day.
If you started with purchased calanoids or eggs,it is just the same,you put them in the jar and fill up to 3L
Well cared cultures seem to last indefinitely,never seem to get old as most other cultures we know.But they can crash sometimes,so 3-4 jars of each species must be kept at all times.
In wild cultures,usually only one species from each bottle (location) develops,but occasionally we can have two or more species together in culture.This is not a problem and in the long run only one species prevails.
In certain times and locations,barnacle nauplii,clam veliger larvae
and other such planktonic organisms come in our wild stock bottles,and thrive in our cultures until they settle and can be removed.
Culture contamination with Artemia and rotifers can always happen in the fish room and we must be very careful to avoid it.Artemia is not a big problem, they will grow and can be removed later.But rotifers will quickly outgrow and kill a calanoid culture.If you see one or two rotifers when checking for nauplii (explained later),you must act immediately and drastically,or you´ll lose all your stocks.You must dispose of the contaminated jar,and wash thoroughly.If it is your only culture and you want to save it,it can be done.You must flush the culture in a 200 mic.sieve with at least 20 L
of water everyday for one week,moving to a clean jar.After that,keep watching until you are sure they were erradicated.
Cultures don´t need any direct light and can be kept at normal fish room temperature.
To check the calanoids best way is with a flashlight playing obliquely from above,against a black background.