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Marine Ornamental Fish & Invertebrate Breeders • View topic - About Ciliates

About Ciliates

About Ciliates

Postby Luis A M » Sun Jul 13, 2008 5:19 pm

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Postby aomont » Sun Jul 13, 2008 11:40 pm

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Postby Luis A M » Mon Jul 14, 2008 11:13 pm

Last edited by Luis A M on Tue Jul 15, 2008 11:27 am, edited 1 time in total.
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Postby KMB » Tue Jul 15, 2008 1:56 am

Here is another side thread where some ciliate culture methods are posted.

http://www.marinebreeder.org/phpbb/viewtopic.php?t=1881

I also have the link to the article that Antony was referring to regarding tangs and ciliates but I can't seem to get it posted. I'll try it on a separate post.

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Postby KMB » Tue Jul 15, 2008 2:15 am

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Postby Luis A M » Tue Jul 15, 2008 11:46 am

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Postby Antony P.M. » Wed Jul 16, 2008 5:40 am

Here is a short description with some biometrical measurements I've found on Amphorellopsis acuta
Quote:
Tintinnid ciliate, the lorica has a pointed, not a truncated, aboral end. The thickness of the wall is variable. Length 76-89 microns, diameter at mouth 29-37 microns, diameter of neck 19- 24 microns, greatest diameter of body 22-27 microns.
Here's the link where I found the info, there's also a pic of another species of the same genus
http://starcentral.mbl.edu/microscope/p ... D=10358559
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Postby aomont » Wed Jul 16, 2008 11:15 am

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Postby KMB » Fri Jul 18, 2008 3:20 am

Just thought I'd share how I've been trying to get different ciliates lately.

Since they always seem to appear in old rotifer cultures I have been setting up buckets of rotifers and feeding them different diets. A few weeks ago I started one with Iso paste and got some ciliates that look like a euplotes that's been cut in half, (so same width as euplotes but half as long). I kept them going until I could borrow some eggs of Opakapaka (Pristipomoides filamentosus) which are very similar in size to Centropyge eggs. I added the eggs to a small 30 liter tank that had these ciliates still being fed on Iso paste. At day 2 post hatch I had approx. 50 ciliates/ml. I stopped adding Iso paste and added live Iso hoping to get a better nutritional profile. On day 3 I checked larval guts which were empty. At the end of day 3 there were hardly any ciliates in the tank. Morning of day 4 I added Iso paste again and by that evening the ciliates had bloomed again but the larval guts were still empty. All larvae were dead by day 6 having had empty guts the whole time.

I think these ciliates were too small. Sorry, I don't have an actual measurement on them. What amazed me though was how fast they responded to changes in diet which could be useful for getting blooms on demand. Also, I was not able to sieve them but I just scooped a little water from the rotifers to start a new ciliate culture and ran it through a 35 micron screen to get rid of the rotifers.

I've let those ciliates disappear mainly because of their size. Haven't had time to start any new batches since but I plan to go back to trying different oily enrichments and see what shows up with those since they should have a good nutritional profile.

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Postby Antony P.M. » Fri Jul 18, 2008 10:53 am

Here is a link to a book on Protozoans in marine aquaculture
http://www.publish.csiro.au/nid/18/pid/2915.htm

and for a glance to a couple of pages in it
http://www.publish.csiro.au/samples/Protozoa.pdf

I still don't have the book, I hope to get it asap.
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Postby Antony P.M. » Fri Jul 18, 2008 11:13 am

Euplotes sp. ciliates have been successfully used, co-fed with S strain rotifers, for the first feeding of the yellow neon goby Gobiosoma evelynae. There is also a PDF on this work.
This is the article I'm refering to
http://www.marinebreeder.org/phpbb/viewtopic.php?t=695
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Re:

Postby Luis A M » Fri Jul 18, 2008 4:08 pm

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Re: About Ciliates

Postby fishyfred » Mon Dec 19, 2011 7:01 pm

Do you guys think it would be possible to separate the eggs using separate pumping systems, which might keep them from cross-contaminating gene pools? I've heard of people using a pump to get the water from a large source--say a pond, and sending it in one direction, while picking up the vitamins through the line like an IV on the other. Might keep the eggs from hatching early, too.
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Re: About Ciliates

Postby irenebl » Fri Mar 09, 2012 1:52 am

although this thread is a little dated its really good, thanks!
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Re: About Ciliates

Postby clark5901 » Sat Jan 26, 2013 3:16 am

Thanks for the nice information in the post...
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Re: About Ciliates

Postby ohadshimon » Sun Feb 09, 2014 4:52 am

Hello
My name is Ohad, and i am part of a team that cultures ciliated protozoa (Conc. of 10k for ml) for fish larvae feeding. our main focus was food fish, such as groupers and bluefin tuna. Recently we decided to test our product on ornamental fish larvae.
My questions to you, who know this world better then me.
What fishes are good to start with (with problems in the larval stage) ?

Best regards
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