Larval L.debelius

Postby aomont » Mon Nov 05, 2007 7:05 pm

The photo are awesome as always Luis !
This variation in time between the zoea (2-7 days) is common or do you think something may have delayed molting ?
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Re:

Postby Luis A M » Mon Nov 05, 2007 7:26 pm

aomont wrote:The photo are awesome as always Luis !
This variation in time between the zoea (2-7 days) is common or do you think something may have delayed molting ?
Anderson.

I think they are taking 4 days between molts.But not all molt at the same time,so I can´t be very precise about that.
I have two Z11 and five Z10 at 35 days.
In Z11 the antenna reaches the fork of the antennula and the length of the scale.Now it has many segments,like a cane:

Image

All five pairs of pleopod buds show:

Image

Closer view:

Image
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Postby Luis A M » Wed Nov 07, 2007 12:46 am

Z12 at 41 days.
Rostrum has now two spines.
Antenna extends beyond the fork of the antennula

Image

Pleopods are more developed and biramous,showing two branches:exopod and the smaller endopod

Image

There are about five setae (hairs) now at the end of the pleopods.They can be seen in this closer view of the 5th pair:

Image
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Postby Luis A M » Sat Nov 10, 2007 1:20 pm

44 days.Found some few hydroid polips.So again took them all and scrubbed the tank clean.Six Z12 and one Z13.
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Postby Luis A M » Sun Nov 11, 2007 3:29 am

Z13 has developed pleopods with the distal third bordered by setae.
Antenna reaches about half the antennula branches.

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Postby FuEl » Sun Nov 11, 2007 1:02 pm

Way to go Luis...Have you tried screening the incoming water? I find that screening the incoming water with a 53 micron mesh helps in preventing hydroids. :)
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Postby chris melb » Sun Nov 11, 2007 9:57 pm

Luis are you using NSW or ASW? has this been aged i.e is it from the Broodstock tank?
Do you filter NSW entering into you larval system when you do a topup etc?
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Postby Luis A M » Mon Nov 12, 2007 1:15 am

Larvae are in a dedicated larval system,ASW,cartridge filter with carbon,UV and head tank that gravity feeds larval tanks.Not practical to screen water coming from the head tank,which must be the hydroid reservoir.
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Postby Luis A M » Wed Nov 14, 2007 12:04 am

48 days.Two Z13,five Z12 :D
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Postby Luis A M » Tue Nov 20, 2007 3:46 pm

54 days,breaking my previous survival record! 8)
Z14,the last larval stage.Pleopods are well developed and functional,both exop.and endop.are wide and paddle shaped,bordered by setae.Endopod has a small appendix interna (AI) which can be better appreciated in a ventral view.Rostrum has two developed spines.
A small hydroid patch was detected,and everything was cleaned up again.
All seven are holding on,albeit with some missing pp5 legs :roll:
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Postby Luis A M » Tue Nov 27, 2007 10:27 pm

Buds of AI are hard to see.Arrows point at them:

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Postby KathyL » Wed Nov 28, 2007 10:00 am

Very exciting!
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I am new to the idea of breeding

Postby Lynx113 » Tue Dec 04, 2007 1:23 am

Very new to the topic, and far away from anything close to starting to breed especially L. Debelius. But in my searching and pondering I found a link that may be beneficial to a lot of you. maybe you already saw it. here it is

http://nsgl.gso.uri.edu/tamu/tamug01003.pdf

Im not on the forums alot so if its useful or not send me an email
Lynx113@juno.com.
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Postby Luis A M » Tue Dec 04, 2007 2:01 pm

Again a hydroid outbreak and one larva died :(
Six remain :roll:
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Postby Sylvio » Tue Dec 04, 2007 2:35 pm

Hi Luis

Tust du deine Nauplien oder Eier desinfizieren?

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Postby Luis A M » Tue Dec 04, 2007 3:19 pm

gar nicht.Das system ist damit kontaminiert.
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Postby mpedersen » Tue Dec 04, 2007 3:46 pm

Luis, forgive me if this question has already been asked / addressed. When you photograph the larvae, are they sacrificed, or do they survive their time under the scope?

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Postby Sylvio » Tue Dec 04, 2007 3:53 pm

Hi Luis

Es muß aber einen Fressfeind geben .
Denn ich hatte sie auch zu 1000 im System.
Vieleicht war der Chelmon der Feind???

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Requested translation:

But there has to be an enemy that feeds on them.
I had them too in the 1000s.
Maybe the Chelmon? was the enemy???
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Postby Luis A M » Wed Dec 05, 2007 2:15 pm

Matt,
I´d never sacrifice anything! :wink:
I put them in a well slide and remove most of the water so they can´t move much.Done gently,this doesn´t affect them.

Sylvio,
Problem with hydroids is that they colonize all available substrates,including inside pipes and hoses where no predator can get them!
I sterilize nests with formalin when I aim for a hydroid free larval tank.
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Postby Luis A M » Thu Dec 06, 2007 12:17 am

This is a better view of the pleopod,where all the parts can be seen! :D

AI=appendix interna
exop=exopod
end=endopod

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Postby Luis A M » Mon Dec 17, 2007 3:52 pm

Another larva died :( It happened short after a power failure,but also before I found a new small hydroid patch :?
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Postby Luis A M » Mon Dec 17, 2007 9:57 pm

Cleaned the tank thoroughly.But another larva doesn´t look OK :(
82 days

Compare the pleopod with previous pic.AI is clearly more developed.Endop.and exop are now completely bordered by setae,about 30.This seems like the final stage yet different,more advanced than the stage shown before :?

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Postby Luis A M » Wed Dec 19, 2007 2:50 pm

It also died :( I now have four 84 day larvae.
Meta should be very close.If only some larva could hold on till then :roll:
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Postby KathyL » Wed Dec 19, 2007 11:48 pm

good luck! :)
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Postby mpedersen » Wed Feb 06, 2008 3:56 am

UPDATES PLEASE!!! Fire shrimp can be done, right? Luis, we're all looking to you to prove the success!!!

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