Larval L.debelius

Larval L.debelius

Postby Luis A M » Sun Sep 30, 2007 2:24 am

I am once more trying to raise L.debelius.Last time was in 2001 when I could only reach 53 days of survival.
I hope I can keep them longer now,hopefully until they settle.I will document all the larval stages.It will be interesting to find out if larval development matches that of peppermints with 11 stages or that of L.amboinensis with at least 14.Please refer to the staging key here in this forum.
So a new run begins.This is Z1,sessile eyes,2.4mm.
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Last edited by Luis A M on Wed Oct 03, 2007 11:44 pm, edited 2 times in total.
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Postby Luis A M » Mon Oct 01, 2007 12:02 am

Z2,four days.2.6mm.
Pediculated eyes,no uropods.
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Last edited by Luis A M on Wed Oct 03, 2007 11:45 pm, edited 2 times in total.
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Re:

Postby spawner » Mon Oct 01, 2007 11:47 am

Exactly the same as L. amb or L. grab. only they settle at 1cm or so.
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Postby FuEl » Mon Oct 01, 2007 9:35 pm

Just below 1cm. The blue lines are exactly 1cm apart. :wink:

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Postby joefrith » Mon Oct 01, 2007 10:31 pm

What are you guys feeding these?
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Re:

Postby Luis A M » Wed Oct 03, 2007 11:34 pm

joefrith wrote:What are you guys feeding these?

I keep with the OTO/bbs scheme.
This is Z3,at 7 days and 3 mm
Uropods show-Two pairs of walking legs.

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Postby joefrith » Thu Oct 04, 2007 12:03 am

So these are large enough to accept bbs right out of the egg?
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Re:

Postby Luis A M » Fri Oct 05, 2007 2:04 am

joefrith wrote:So these are large enough to accept bbs right out of the egg?

Sure.See "crustacean larviculture" :D
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Postby Luis A M » Sat Oct 06, 2007 1:53 am

Z4 at 9 days and 3.2mm
Pereiopod 5 shows.They are uniramous (one branched),large and ended in paddles.Typical of Lysmata larvae.

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Postby FuEl » Sat Oct 06, 2007 8:05 am

Nice photos Luis, can't wait for the larvae to turn purplish-red. They are most beautiful around that time. :D
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Postby Luis A M » Sun Oct 07, 2007 10:14 pm

Thanks Junkai,how long yours took to meta?

Z5 at 11 days and 3.5 mm
Three pairs of walking legs:
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easier to see in a lateral view:

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Postby FuEl » Mon Oct 08, 2007 10:19 am

Hi Luis, somewhere just before 60 days. Think it was between 55-60 days at a temperature of 27 degrees celcius. I fed them alot of junk food besides Artemia. The settlement rate when I tried was'nt that high (somewhere around 5 or 6 individuals), as I kind of mistreated the larvae in the beginning due to my attempts with L. amboinensis. :)
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Postby Luis A M » Fri Oct 12, 2007 1:37 am

Some days ago hydroids were found :x I am siphoning them every two days,but sooner or later I will have to move the larvae to a clean tank :?

Z6 at 15 days and 3.6mm
Four pairs of walking legs,the red tipped ones.

Image

focusing on the legs:

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and a side view:

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Postby Noof » Tue Oct 16, 2007 7:35 pm

As I understand Luis you change water on these guys every couple of days? How much and how often?

100% every three days?
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Postby Luis A M » Wed Oct 17, 2007 2:04 am

I am mowing hydroids every two days.
Tank is in a system.No water changes other than replacing siphoned water,and compensate for evaporation.
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Postby Luis A M » Thu Oct 18, 2007 3:24 pm

TRAGEDY AGAIN! :oops:
Hydroids are winning the battle,they regrow faster that the every two day mowing.
When larvae molted to Z6,they looked not so many.I could see about 50 of them.Now they molted to Z7,at 20 days,and I had a massive die off.
So I scooped out the survivors,(only freaking eight :twisted: ) and emptied and cleaned the tank.
Playing again the stupid little indians game! :(
Always larval mortality coexists with hydroids bloom.Don´t want to be paranoic but suspect both facts are related.As my system is contaminated with hydroids,it will be a recurrent problem unless I shut off and sterilize the whole system.But because Lysmata larvae take up to 5 months to settle,I must compromise with a less radical aproach :roll:
Ups and downs all the time!.You can´t get bored with this hobby! :x
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Postby Luis A M » Thu Oct 18, 2007 7:39 pm

Anyway,this is Z7 at 20 days and 4mm

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The larva has now all five pairs of walking legs:

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Now we´re getting into the unknown :? Will Z8 show the development of the antenna,like L.amboinensis,or will it show pleopod buds,like the peppermints?.See the staging table.
I bet it will follow the stages of L.amboinensis.For several reasons I believe these two groups are next of kin.Andy R.disagrees here :wink:
Just in case,I'm posting a close up of the head of Z7 showing the antenna´s buds:

Image

Going from the middle line to the sides,one can see A1 (the antennula),the scale of A2 and the eye.The bud of the antenna (flagellum of A2) can be seen between A1 and the scale.
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Postby KathyL » Thu Oct 18, 2007 11:04 pm

absolutely awesome!

Luis, what else do you have on that system? If it's not just shrimp, surely you could cycle a new aquarium to put them into later.....
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Postby Luis A M » Fri Oct 19, 2007 2:06 am

Thanks Kathy :D
Mostly larval shrimp tanks,with occasional larval fish tanks being hooked up.I could stop the circulation and chlorinate the head tank for one hour or remove the bioballs and sterilize the whole system shutting larval tanks circulation,of course.
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Postby Luis A M » Tue Oct 23, 2007 12:50 am

Z8,at 24 days and 4.7mm

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As expected, larval development of L.debelius follows the same stages of L.amboinensis.See the antenna reaching the setae of the scale (arrow)

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and a closer view:

Image
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Postby mpedersen » Fri Oct 26, 2007 4:58 pm

I have to tag on to this one - YOU CAN DO IT LUIS!!!

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Postby quantim0 » Fri Oct 26, 2007 5:25 pm

The other grad student in my lab worked with L. debelius for her undergrad research. From what I have gathered from her, she had 1 settle out at about 120 days and the rest took at least 150-180. She's thinking of continuing with them for her grad thesis, but she's unsure about working with a species with such a long larval stage.

Good luck with your babies, hope they settle for you.
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Re:

Postby FuEl » Sat Oct 27, 2007 5:37 am

quantim0 wrote:The other grad student in my lab worked with L. debelius for her undergrad research. From what I have gathered from her, she had 1 settle out at about 120 days and the rest took at least 150-180. She's thinking of continuing with them for her grad thesis, but she's unsure about working with a species with such a long larval stage.


Hi quantim0,

That sounds awfully long for L. debelius (twice the reported durations). Such a larval duration sounds to point to L. amboinensis instead. The post-settlement size for L. debelius is only around 8mm.
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Postby Luis A M » Fri Nov 02, 2007 6:25 pm

Z9,at 31 days.Antennae are longer and divided in two segments.This can be seen in a close up of the head:

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Postby Luis A M » Mon Nov 05, 2007 2:34 am

Z10 at 35 days.Seven larvae remain.
Antenna is about half of the scale and shows several segments:

Image

Pleopod buds begin to show,but not the first pair.This one has buds of pairs 2,3,4 and 5,with the fifth bud more developed:

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This one has only buds of 3rd and 4th pairs:

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While this other one has all 2,3 4 and 5:

Image
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