[i]Elacatinus oceanops[/i] breeding log - Dr Hsu

Re:

Postby DrHsu » Fri Feb 15, 2008 12:02 am

Luis A M wrote:
DrHsu wrote:
danch wrote:How are you taking these pictures? Macro mode and a steady hand? Flash or no flash?

I'm using a Nikon D70 with 60mm micro, SB-800 flash. Handheld with some support from a stool. Synch in manual at 1/500 and f22. Prefocus to max magnification and wait for the larvae to come into view, and adjust focus by moving back/forth as required. A tripod would be better but too much trouble to set it up! :)

You hand hold the flash too?Aiming from the top or angled from the front?
Luis, the flash is mounted on the camera's hotshoe, shooting straight at the tank. At this distance, it seems you don't get the irritating flash reflection on the front glass. Would be better with top down flash and a weaker back flash....but would be too much trouble to set up for a few shots...
Li Chieh

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Re:

Postby DrHsu » Fri Feb 15, 2008 12:05 am

KMB wrote:I somehow missed seeing this thread. Came upon it today and it's great!

Was it day 8 when you started adding the Artemia? Would you consider doing a run with 24hr lighting so that you can feed twice a day. It would be interesting to see if additional feedings will help them through day 13.

Karen
Hi Karen,

I started feeding on Day 9 - fed in the morning and took pictures a few hours later.

Due to work, I generally only get to feed once a day which is in the mornings. On the days that I am at home, I may feed another time but with such a small spawn, there was usually lots and lots of left over rots that it was unecessary. A 24 hr lighting regime might work, but I would be too dead to do anything :D
Li Chieh

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Day 15 pictures

Postby DrHsu » Sat Feb 16, 2008 10:24 am

I'm down to about maybe 6 now. A few are looking quite nice but the rest are looking like they're not growing much. Swimming also seems to be more "larval like" - spurts of swimming upwards; doesn't look too good :cry:

Trying a little dry feed today as well....

First, a top view - slightly different from the other days (Pics are no longer 100% crops - the larvae are too large to fit 800 pix length)
Image

Some lateral shots...
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Li Chieh

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Day 16 pictures

Postby DrHsu » Sun Feb 17, 2008 12:53 pm

:D Seems like I still have 12 larvae! And they are looking better than yesterday. Probably miscounted yesterday when they were a bit stressed out. Perhaps the more than 50% water change helped :D

Image

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Postby Gouldian » Tue Feb 19, 2008 10:31 pm

Congrats! Free this Thursday? :D
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Postby mpedersen » Fri Feb 22, 2008 4:24 am

Any new developments? Blue Stripes yet?

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Re:

Postby Luis A M » Fri Feb 22, 2008 7:05 pm

mpedersen wrote:Any new developments? Blue Stripes yet?

Matt

Black stripe comes first :D They reminded me of Barbus titteya at that stage 8)
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Day 22 pictures

Postby DrHsu » Sat Feb 23, 2008 1:08 pm

Sorry about the slower updates....work and life got in the way :D

Went down from about 12 to about 3 or 4 almost overnight at around day 18 or so. Not too sure what happened but I think it could have been the lack of water change..... :oops:

As of today (day 22) I only have 2 left. One looks "better" than the other. Hopefully at least one will make it to settlement!

Also trying to figure out this new cam of mine so somehow the pics don't seem to be as nice :? Gimme a bit of time to figure it out :o

Image

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Day 23 and day 27 pictures

Postby DrHsu » Thu Feb 28, 2008 11:54 am

Here's a pic of one of the larvae at day 23

Image

As of this evening (day 27), there are still 2 larvae. No coloration coming in yet....Looks like they are eating well, tho. Just hope that my attempts at enriching BBS will get them through to settlement....

Image

Image
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Postby mpedersen » Thu Feb 28, 2008 12:00 pm

Lookin' good there Li Chieh!!! AWESOME pictures!

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Postby Luis A M » Thu Feb 28, 2008 2:18 pm

Indeed your pics are so good that seem irreal :shock: And you take it just thru the front glass from your tank! :shock:
Meta is at the turn of the corner,see the flexed notochord.Expect it at 30-35 days.Watch for the black stripe and/or initial clinging to walls.
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Postby FuEl » Fri Feb 29, 2008 1:23 am

I simply love the last shot. Incredible detail.. :shock:
I love Artemac!
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Postby DrHsu » Fri Feb 29, 2008 4:41 am

Thanks for the nice comments: just a couple of lucky shots :D Bought a new clip on compact flourescent light that was on sale and used it for lighting the tank while taking pics - when the larvae positioned themselves just right under the light, sometimes I can get good pics like this. Otherwise they're just a blurry mess :lol:

Can't wait for settlement - another few days, hopefully! Already, one was noted to be hugging the bottom silicon seam for a while and I thought this was going to be it....but it later went back to mid water swimming :(
Li Chieh

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Postby till » Fri Feb 29, 2008 12:14 pm

Already, one was noted to be hugging the bottom silicon seam for a while and I thought this was going to be it....but it later went back to mid water swimming Sad


mine started to settle at day 26, as stated in the Breeders Guide. first they get black dots at the tail as seen in your fantastic pictures ;) then black stripes a few days before settlement. when kept in groups they stay free swimming most of the time even after metamorphosis

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Postby Luis A M » Fri Feb 29, 2008 1:18 pm

Li Chieh,these pics show the backlit effect Witt gets with his sophisticated (and expensive)set-up! 8)
It would help readers if you put the age of the larvae at the beginning of each post :wink:
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Postby DrHsu » Fri Feb 29, 2008 8:29 pm

Luis,

With the clip-on light, the backlighting effect can happen when the larvae is in front of the bulb, which is what I believe happened in these cases. It shows up the transparent outlines much better this way. I ordered some ebay wireless flash triggers and hopefully will be able to get better shots after some experimentation.

If you look at the subject field on top of each posts, I have included the age of the larvae there.....

Till: I noticed the black dots quite a number of days ago. IIRC around 20 days (have to look at the pics to confirm) or even less in some of the larvae.

Anyone noticed the bright red spot at the base of the pelvic fin? Interesting..not sure if it really is a pigment color or just a reflection from the overhead lighting. Hopefully it isn't a hot pixel in my camera sensor :lol:
Li Chieh

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Postby David M » Fri Feb 29, 2008 10:16 pm

RGreathouse just had some settle at 22 days, heater malfunctoned and they were at 88f I think he said. Maybe I can get himto post here. Mine are at only 7 days and probably 50% mort so far.
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Postby DrHsu » Fri Feb 29, 2008 10:48 pm

I just had a look at them and one is definitely hugging the walls, behaving in a normal neon goby style - "hopping" from one spot another.....but....still transparent!
Li Chieh

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Day 30 Pictures - META!!

Postby DrHsu » Sun Mar 02, 2008 12:27 am

I have METAMORPHOSIS!!! :D

Only one has settled, the other still transparent but likely will follow suit in a day or so. The little bugger is pretty fast and his favourite spot is in the back corner farthest from the front glass :x so it was tough to even get a half decent picture....but...I couldn't wait :lol:

Image

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Matt and Luis were both right - blue and black both appeared at the same time.... :D
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Postby Mr Miagi » Sun Mar 02, 2008 1:23 am

Congratulations DrHsu, that is awesome! :D
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Postby FuEl » Sun Mar 02, 2008 3:18 am

Such dramatic change. :shock: Very nice!
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Postby mpedersen » Sun Mar 02, 2008 12:21 pm

WHOO HOO! Congratulations that is AWESOME!!! SO PRETTY!

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Postby Luis A M » Sun Mar 02, 2008 4:11 pm

That´s weird,the black stripe always shows before the blue one (or yellow,in E.figaro).Perhaps the blue reflection is brought out by the flash?
Congrats,of course! :D
Luis
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Postby David M » Sun Mar 02, 2008 6:47 pm

Pretty work, congrats. :D
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Postby KMB » Mon Mar 03, 2008 3:21 am

That's so exciting! I'm very happy for you!!!

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