Green Isochrysis

Green Isochrysis

Postby KathyL » Tue Jun 14, 2011 8:14 pm

Hi all,
Years ago I had a thriving Isochrysis culture. It sat in a 2 L soda bottle next to lots of bottles of nanochloropsis. When I went on vacation, my fish sitter saw the brown bottle, and assuming it was dead, turned off the air. By the time I got back, it really was dead. I switched to frozen phyto, as I really did not need live.

Now I'm trying to culture live phyto again. Jim W kindly sent me a thriving C Iso culture. I tried starting it. It did not crash, but took a week to really bloom. Unfortunately it has bloomed green….

This happened once before to me, and the bottle eventually turned brown again. Unless my memory fails me, which is happening more and more lately.

Does anyone have any experience with this phenomenon? Is high temperature a cause?
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby JimWelsh » Tue Jun 14, 2011 11:36 pm

Kathy,

Not sure what is going on with your starter. I'm only culturing Iso right now, so it is unlikely a Nanno contamination. I've been culturing it at between 70-75 F.

Jim
User avatar
JimWelsh
Read-Only
 
Posts: 1806
Joined: Sat Sep 19, 2009 11:54 am
State/Region: California
Country: US

Re: Green Isochrysis

Postby KathyL » Wed Jun 15, 2011 9:17 am

Yeah, My basement is at 78 right now. I'm going to keep it going and see what happens. I don't have nanno or any other phyto growing now either, except for the stuff on sides of the aquariums. I wasn't thinking that there was anything wrong with the culture you sent, just the conditions in my basement.:)
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby Luis A M » Wed Jun 15, 2011 2:58 pm

Hi,Kathy,great to see you again,you´ve been missed! :|
Whenever a culture shows a funny shade,I check it under the scope;you´ll usually find Chlorella or other feral contaminant. :wink:
Luis
User avatar
Luis A M
Moderator
 
Posts: 5630
Joined: Sun Mar 04, 2007 2:34 pm
Location: Buenos Aires,Argentina

Re: Green Isochrysis

Postby BaboonScience » Wed Jun 15, 2011 11:46 pm

Hey, Kathy,
I currently have a culture of tIso that I am fairly certain is clean. It turned sort of a "straw green" during a prolonged power outage and temperature drop last winter. Given more acceptable environmental conditions, it has remained the same "shade", indicating that those accessory pigments still predominate. The organism is identical to the other tIso cultures under the microscope. Could it be that the accessory pigments selected under stress remain in dominance when the stress is removed? For the organism, there would be no problem with this until the next stressor comes along.
BTW, looking at it right now, it is still straw green. The tIso cultures beside it are golder brown (Algagen cultures). These cultures have been side by side for greater than two months. Perhaps, a stress is required before you see an accessory pigment shift?

John
"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)
User avatar
BaboonScience
 
Posts: 3629
Joined: Fri Mar 02, 2007 8:47 pm
Location: High Shoals

Re: Green Isochrysis

Postby KathyL » Thu Jun 16, 2011 12:13 am

How much magnification do I need to see Isochrysis and what should it look like. Swimmers? With my home microscope I can barely see O. marina….

By the way, nice to see the two of you as well. Going to Macna this year?
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby JimWelsh » Thu Jun 16, 2011 1:27 am

O. marina are about 15 x 30 microns. Isochrysis is 5 - 7 microns, more or less round in shape, and motile (although they do not move anywhere nearly as quickly as O. marina).
User avatar
JimWelsh
Read-Only
 
Posts: 1806
Joined: Sat Sep 19, 2009 11:54 am
State/Region: California
Country: US

Re: Green Isochrysis

Postby KathyL » Thu Jun 16, 2011 9:25 am

This morning there was a green precipitate. I've got contamination. Time to order from Algagen.
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby Luis A M » Thu Jun 16, 2011 3:20 pm

Kathy,I considered attending Macna,but couldn´t convince the family about visiting Iowa! :mrgreen: BTW,they loved that city tour of SL.
T-ISO are ovoid/roundish and motile,you can see that even under low magnif.Chlorella are large,green,round and non motile.
Permanent loss of colour of T-ISO,as noticed by John? Hmmm,something along these lines is frequently reported by those attempting to culture RHO.It turns green.I consulted Gary W,a well known authority,who said that the cells must be dying and losing the red pigment while still retaining the green ones.Hmmm again :?
This reminds my visit to a dear friend and famous clown breeder in Chicago.He showed me his T-ISO culture and it was completely green! :shock: My friend is daltonic and couldn´t see the green colour!
His hatchery was visited by virtually every breeder coming to the area;funny that nobody told him before...
Not relationed with colour,but I have seen loss of motility in some motile algae.Other than that,they remain alive and reproduce normally.In one case,a T-ISO,it was clearly produced by thermal stress.
Luis
User avatar
Luis A M
Moderator
 
Posts: 5630
Joined: Sun Mar 04, 2007 2:34 pm
Location: Buenos Aires,Argentina

Re: Green Isochrysis

Postby BaboonScience » Thu Jun 16, 2011 11:33 pm

Luis
The "green" tIso is really a yellow/green. I keep a 250 ml flask of it beside the other tIso cultures (classic golden brown). Obviously, side by side, the environmental conditions are nearly identical. The loss of pigmentation was certainly due to a temperature spike. Interesting that it persists.

Kathy, not sure, but I think that our travels may be in the opposite direction MACNA week.
John
"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)
User avatar
BaboonScience
 
Posts: 3629
Joined: Fri Mar 02, 2007 8:47 pm
Location: High Shoals

Re: Green Isochrysis

Postby Luis A M » Fri Jun 17, 2011 2:57 pm

John,interesting observation.The "greened"RHO also remains green for good. :?
I failed to imagine what "straw green"meant.Which is easy;straw is yellow,straw green had to be yellowish green! :mrgreen:
Which reminds me of the old Spanish song "Ojos Verdes"(Green eyes):
Ojos verdes,verdes como la albahaca (green as the basil)
Verdes como el trigo verde (green like the green wheat)
y el verde,verde limon (and the green,green lemmon)

Excuse the disgression :oops:
Luis
User avatar
Luis A M
Moderator
 
Posts: 5630
Joined: Sun Mar 04, 2007 2:34 pm
Location: Buenos Aires,Argentina

Re: Green Isochrysis

Postby BaboonScience » Fri Jun 17, 2011 3:12 pm

Yes, I keep thinking that it will eventually recover the accessory pigments. That is why I keep that culture running.

And thanks for the Spanish lesson. I think that my comprehension is improving with your assistance. 8)
John
"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)
User avatar
BaboonScience
 
Posts: 3629
Joined: Fri Mar 02, 2007 8:47 pm
Location: High Shoals

Re: Green Isochrysis

Postby Luis A M » Sat Jun 18, 2011 12:49 pm

BaboonScience wrote:

And thanks for the Spanish lesson. I think that my comprehension is improving with your assistance. 8)
John

HEHE,was it so obvious that I was spamming some Spanish lessons :lol:
Now all Mofibers know how to say "Green" in Spanish.And Portuguese,and Italian! 8)
Luis
User avatar
Luis A M
Moderator
 
Posts: 5630
Joined: Sun Mar 04, 2007 2:34 pm
Location: Buenos Aires,Argentina

Re: Green Isochrysis

Postby KathyL » Sat Jun 18, 2011 8:29 pm

Interesting:
I left my culture bubbling, and now it is a decisive cloudy yellow, almost golden. I wonder if the green stuff is converting back to brown, or if the Iso is outgrowing the green "contaminate" that appeared to crash on the bottom. I'll let it go, and we'll see what happens.

Jim, I've been giving my little culture of A panamensis a daily dose from the O marina jug, and the A. p. seems to have bloomed as well. I see the little guys twitching around in the culture, when I put my glasses on. I can see the O. marina under the scope, and it looks appropriately dense. I'm very happy. Thanks for your help.
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby JimWelsh » Sun Jun 19, 2011 4:02 am

Glad to hear it! How are the Moina salina doing? If you can get the Iso to thrive (and it sounds like you are well on your way) then all the others will thrive, too! Good luck, and please keep us posted.
User avatar
JimWelsh
Read-Only
 
Posts: 1806
Joined: Sat Sep 19, 2009 11:54 am
State/Region: California
Country: US

Re: Green Isochrysis

Postby KathyL » Sun Jun 19, 2011 2:05 pm

not sure about the moina salina. They seemed quite robust at first, but now I'm thinking the A panamensis is doing better.
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby AquaticEnterprises » Wed Jun 22, 2011 8:52 pm

So...who's got the time and ability to be a kind soul and send me a small starter of Iso? The batch I got this last go round did absolutely nothing. I'd like to get it going continuously in order to feed my Parco copepds (which since my Iso never took off at all, it looks like I'll need to get a starter culture of them again as well once I get the hang of this). Anyone? Bueller?
Fred
AquaticEnterprises
AquaticEnterprises
 
Posts: 103
Joined: Fri Mar 02, 2007 7:28 pm
Location: Neenah, WI

Re: Green Isochrysis

Postby JimWelsh » Thu Jun 23, 2011 12:10 am

I could, but you'd get a MUCH better (cleaner) starter by ordering one from SeahorseSource.com. That's where I get mine from.
User avatar
JimWelsh
Read-Only
 
Posts: 1806
Joined: Sat Sep 19, 2009 11:54 am
State/Region: California
Country: US

Re: Green Isochrysis

Postby AquaticEnterprises » Thu Jun 23, 2011 9:30 am

Jim, any idea how much shipping from them generally runs on phyto cultures? I'm working on a limited budget here. Would you recommend T- or C-Iso?
Fred
AquaticEnterprises
AquaticEnterprises
 
Posts: 103
Joined: Fri Mar 02, 2007 7:28 pm
Location: Neenah, WI

Re: Green Isochrysis

Postby KathyL » Fri Jun 24, 2011 8:19 pm

OMG moina salina may be taking over the basement. Turns out they like Oxyrrhis marina.

Iso: Last week I took the bottle with the crashed green stuff at the bottom and the golden stuff circulating, and decanted off enough golden to start a new bottle. Again, it took some time to bloom, and now its the brightest green you've ever seen. I give up. I may just dump this in the saltwater tubs I am starting in the backyard and order some T-Iso fresh.

Jim, what's the difference between T Iso and C Iso?
User avatar
KathyL
 
Posts: 3442
Joined: Wed Feb 28, 2007 12:13 am
Location: Missouri, USA

Re: Green Isochrysis

Postby JimWelsh » Sat Jun 25, 2011 1:20 am

@AquaticEnterprises: Ask them about the shipping.

@Both: The main difference, as I conclude from the description on the SeahorseSource.com website, is that C-Iso is more temperature tolerant. That's about it. I interpret that to mean that it will do better at warmer temps than T-Iso.
User avatar
JimWelsh
Read-Only
 
Posts: 1806
Joined: Sat Sep 19, 2009 11:54 am
State/Region: California
Country: US

Re: Green Isochrysis

Postby AquaticEnterprises » Sun Jun 26, 2011 10:47 pm

Thanks Jim, I sent Dan an email last week and got my answer. They seem fairly reasonable.
Fred
AquaticEnterprises
AquaticEnterprises
 
Posts: 103
Joined: Fri Mar 02, 2007 7:28 pm
Location: Neenah, WI

Re: Green Isochrysis

Postby NirvanaFan » Wed Jul 13, 2011 9:05 pm

Mind if I ask what he quoted you in shipping? I am looking for a good starter culture.
NirvanaFan
 
Posts: 66
Joined: Wed Jul 14, 2010 4:55 pm

Re: Green Isochrysis

Postby BaboonScience » Wed Jul 13, 2011 10:25 pm

I recently ordered and shipping was very reasonable = low.
John
"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)
User avatar
BaboonScience
 
Posts: 3629
Joined: Fri Mar 02, 2007 8:47 pm
Location: High Shoals

Re: Green Isochrysis

Postby Suzy » Thu Jul 14, 2011 9:45 am

I ordered from Dan recently, too. Great service and ti came quickly. Plus, the price was very reasonable.
www.Suzysreef.com
User avatar
Suzy
Read-Only
 
Posts: 2190
Joined: Sun Dec 20, 2009 6:54 pm

Next

Return to Phytoplankton



Who is online

Users browsing this forum: No registered users and 7 guests

cron