Kathy,I considered attending
Macna,but couldn´t convince the family about visiting Iowa!
BTW,they loved that city tour of
SL.
T-ISO are ovoid/roundish and motile,you can see that even under low magnif.Chlorella are large,green,round and non motile.
Permanent loss of colour of
T-ISO,as noticed by John? Hmmm,something along these lines is frequently reported by those attempting to culture
RHO.It turns green.I consulted Gary W,a well known authority,who said that the cells must be dying and losing the red pigment while still retaining the green ones.Hmmm again
This reminds my visit to a dear friend and famous clown breeder in Chicago.He showed me his
T-ISO culture and it was completely green!

My friend is daltonic and couldn´t see the green colour!
His hatchery was visited by virtually every breeder coming to the area;funny that nobody told him before...
Not relationed with colour,but I have seen loss of motility in some motile algae.Other than that,they remain alive and reproduce normally.In one case,a
T-ISO,it was clearly produced by thermal stress.