The "Blue Tooth Walrus" that Johnrt designed will help me re-use culture water because I do live in a sub-division and have a septic tank so I will be unable to continually dump the used water.
sunny d polyp wrote:. . .and a sand filter (fluidized bed), . . .
johnrt wrote:sunny d polyp wrote:. . .and a sand filter (fluidized bed), . . .
rayjay wrote:Why not just buy the phyto paste then?
You eliminate cost of lighting, containers, and your time.
It takes very little to do a lot.
Zante wrote:
How is it possible though that contamination happens more quickly every time I sterilise the reactor?
robvdv wrote:just 1 more question - is there a timing for boiling, like 3 or 5 mins?
I do 7 minutes per litre in my microwave. It doesn't boil (not necessary) and it seems to get very hot. Hot enough to kill pretty much anything, IMO. I usually microwave it in a glass jug with a dinner plate on top. The dinner plate reduces contaminants falling in the water as it cools.
With regards chlorinating/dechlorinating, I found it frustrating while researching this to find "the truth", but this is what I use now:
30 minute sterilize: 1.6ml 5.5% household bleach per litre
6 or more hour sterilize: 0.5ml 5.5% household bleach per litre
To dechlorinate, I use a solution of 55g/litre sodium thiosulphate. 1ml thio dechlorinates 1ml bleach at these concentrations.
When I want something really sterile, I microwave container and liquid together, as Andy says. Otherwise I use chlorine. I shake the vessel a few times during the chlorination process to spread out the chlorinated fluid across all surfaces.
I have a question for the gurus. What sterilizes the thio solution? I guess it's self-sterilizing, but I haven't seen anyone explicitly say this anywhere. I think I've seen references to thio being used as a sterilizer in its own capacity, but maybe someone can confirm?
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