Flasks
Stock cultures are kept pure and possibly sterile in flasks. Each flask is filled with 60 ml of medium, cotton stopped and sterilized in the microwave oven. When cool, 10 ml of a thriving culture is seeded with a sterile pipette.
I keep three flasks for each algal species; old, medium and new. I seed new cultures 2-3 times per month, so this is not too much work. Every morning I agitate the cultures by swirling.
I need to keep stock cultures because it is very difficult to obtain them here. In places where they can be easily replaced when lost, one can do without them.
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