Tetraselmis Culture (my first phyto culture attempt)

Re: Tetraselmis Culture (my first phyto culture attempt)

Postby EasterEggs » Mon Apr 02, 2012 11:13 pm

John, it is Ms Eggs! :p I haven't got that air filter going yet...I need to buy a tap and die so I can install my threaded airline fittings into the pvc caps.
Given sufficient thrust, pigs will fly just fine.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby EasterEggs » Tue Apr 03, 2012 11:42 am

So pop bottle #1 was started on March 25th with about a 350 mL dark starter so it is 9 days old today and has been very dark (can't see through it) for several days now. This morning it suddenly settled out real bad so that I could just barely kind of see through it. I assume this is the start of a crash?

I poured a third of the bottle down the drain (no empty pop bottles), and topped back up with sterile medium. I will get more pop bottles today so I don't have to waste the phyto when I'm trying to ramp up! That was annoying dumping it down the drain although now I think about it I probably could have saved it in a bucket for the day. Argh.

In the future, when a phyto culture settles out like that (assuming this is the start of a crash), should I only split the phyto that is still motile? Should I toss the bottom of the bottle that settled?
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby rayjay » Tue Apr 03, 2012 6:03 pm

If it settled out it would be my guess that being no longer motile, it is dead.
I would then have kept what was still in solution assuming it was motile and still alive, and discarded the settled portion.
That being said, tetra isn't one that I've grown myself.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby Luis A M » Wed Apr 04, 2012 1:09 am

Not necessarily.Since many years I keep a non motile strain of TET,from Karen B.of Hawaii.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby rayjay » Wed Apr 04, 2012 10:24 am

But if the strain was motile to begin with, is it likely to change to a non motile strain?
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby EasterEggs » Sat Apr 14, 2012 10:07 am

I'm still having decent success with these cultures. I seem to have to split them around 6 days or the alga will fall out of the water column on day 7 or 8. I find the cultures get very dark within just a few days after a split using 30% alga culture and 70% growing medium.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby Luis A M » Sat Apr 14, 2012 12:14 pm

rayjay wrote:But if the strain was motile to begin with, is it likely to change to a non motile strain?

I have seen it happen after thermal stress,though the condition reversed after some time.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby JulioC » Tue Apr 17, 2012 10:27 am

Today something similar happened to me.
I had a Tet culture for 10 days or so and I was using abaut 5/10 percent each day replacing culture water with growing medium.
In a peiod of 5 hours it went from dark green to light green but looking inside I only see small dots of algae. It is like all the algae made small dots of algae witm 1/2 mm of diameter aprox (colour of "balls"is dark green).
I tried to filter the water with a cofee filter but the result was almost cristal clear water... (When the culture is Ok I filter it and and only detritus remains in the cofee filter).

Should I split them earlier as EasterEggs? I usded to keep some bottles for abaut 20 days without problems.

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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby EasterEggs » Tue Apr 17, 2012 11:19 am

I also have to "swirl" the cultures twice a day or the alga will fall out of the water as well. I think it wants stronger aeration, but I can't do that right now.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby JulioC » Tue Apr 17, 2012 5:34 pm

Yes, I have heavy aereation and that improved things a bit and I don´t have sediments untill culture crash. Is is not a gradual process.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby mmoner » Sat Apr 28, 2012 9:44 am

Same thing happened to me as my second batch of TET became non-motile under microscope.

After few hours of harvesting, settles down as in the picture.

Image

I've read that with too much f/2 or fertilzer, they might become non-motile, but would not be a problem.

Any ideas? what else could effect the motility and could that be reversed ?

Thanks...
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby Luis A M » Sat Apr 28, 2012 12:45 pm

Luis A M wrote:Not necessarily.Since many years I keep a non motile strain of TET,from Karen B.of Hawaii.

The good news is that there is nothing bad with a non motile strain,it just needs strong aeration :wink:
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby mmoner » Sun Apr 29, 2012 12:40 pm

And this is next morning, is this normal since TET is heavier than NanoCl.

I never see that in my NC cultures.

How should we store TET reserves then?

Image
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby Luis A M » Sun Apr 29, 2012 6:52 pm

mmoner wrote:And this is next morning, is this normal since TET is heavier than NanoCl.

I never see that in my NC cultures.

How should we store TET reserves then?


What you have in mind for storing reserves? :?
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby mmoner » Tue May 01, 2012 4:16 am

I can keep NC for a month refrigirated. Just that settling of TET confuses me.

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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby Luis A M » Tue May 01, 2012 10:43 am

Actually,this could make concentrating your algae easier.So you save the room needed for storing water. :wink:
Have you checked with a microscope that they are still TET?.
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Re: Tetraselmis Culture (my first phyto culture attempt)

Postby mmoner » Tue May 01, 2012 2:41 pm

Luis A M wrote:Actually,this could make concentrating your algae easier.So you save the room needed for storing water. :wink:
Have you checked with a microscope that they are still TET?.


Agree with the concentration idea :)

Yes they are still Tet(non-motile)but i also saw an Euplote in it.
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