hi guys! im culturing
nannochloropsis using
2 liter bottles and a few more sizes. I clean the bottle with bleach then microwave them for 15 seconds. i clean them every after 7 days before innoculation. if the bottle is very dirty, i change it. after a few tries like 3-4x, my culture grow brown algae. then eventually crash. what seems to be the problem? where are those diatoms coming from? i use 2x 40w flourescent bulbs 16 on 8 off using BOILED natural sea water. another problem is, after a few tries, my
nanno wont turn as green as before. they start turning yellowish green then eventually crash too. is it because im using
NSW? i boil all so all organisms die. is it better to use rock salt +
RO water rather than
NSW? i use coffee filters to remove the solid particles before innoculating the
nanno to a new container.