Phyto culturing.Why,Which and How.

A bone! :lol:

Postby BaboonScience » Sun Oct 25, 2009 10:47 pm

Ok, the other stickie up there is a less than comprehensive list of Algae Culture and Identification sites.
viewtopic.php?f=145&t=124
I have tried in the past to update this list but have let it go in the past few months.
If anyone finds other sites, Let me know and I will add them.

I also need to organize that list into appropriate categories.
John
"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)
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Re: Phyto culturing.Why,Which and How.

Postby JimWelsh » Sun Oct 25, 2009 10:54 pm

Thanks, John, but I've clicked on all of the links in that sticky, and most of them are now broken. Once again (hawklover, are you listening?) I'd be willing to work with others on organizing this info in a useful, approachable format, FWIW.
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Re: Phyto culturing.Why,Which and How.

Postby BaboonScience » Mon Oct 26, 2009 8:52 am

JimWelsh wrote:Thanks, John, but I've clicked on all of the links in that sticky, and most of them are now broken. Once again (hawklover, are you listening?) I'd be willing to work with others on organizing this info in a useful, approachable format, FWIW.

Thanks Jim.
Most of those sites still exist. I'll try to fix them today.
But this points out a problem with working with links to other sites and internet information. Sometimes the linked site just disappears. We really should try to consolidate the How too information and tables here if possible.
John
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Re: Phyto culturing.Why,Which and How.

Postby hawklover » Wed Oct 28, 2009 6:29 pm

Jim,
I am here and still listening! If I can be of any help at all, please let me know. I am a hands on learner and have to read things over and over unless I have it right in front of me. Let me know what I can do, I would love to help!
Ben (P.S. I really, really, really want to be the first person to succsesfully breed hawkfish)
Broodstock: Flame Hawks, Engineer Gobies, Ocellaris Clowns, Bangaii Cardinals, FireFish Magnifica, Azure Damsels, more to come with time!
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Re: Phyto culturing.Why,Which and How.

Postby WDT » Sun Jun 06, 2010 11:07 am

JimWelsh wrote:Can anybody post a table showing optimum SG, Temperature, Lighting, Media strength, etc., for the more "finnicky" algaes, such as Pavlova, Rhodomonas, etc.?


Is this located some were on the site, I cannot seem to locate it on this site or any were else for that matter.

Thanks
Will
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Re: Phyto culturing.Why,Which and How.

Postby JimWelsh » Sun Jun 06, 2010 11:09 am

Actually, this project never happened. I was thinking about this just the other day. Perhaps it's time to resurrect the idea!
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Re: Phyto culturing.Why,Which and How.

Postby WDT » Sun Jun 06, 2010 11:41 am

Let me know how I can help.
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Re: Phyto culturing.Why,Which and How.

Postby JimWelsh » Sun Jun 06, 2010 12:03 pm

Gather data. Search online sources and books for the answers to these questions, so we can build the table of information.
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Re: Phyto culturing.Why,Which and How.

Postby jolson » Sun Jun 06, 2010 5:02 pm

glad to see this subject coming back up. I will be watching for any new information. This is a subject that will be of help to anyone trying to breed fish or inverts of which I am one
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Re: Phyto culturing.Why,Which and How.

Postby Suzy » Sun Jun 06, 2010 5:09 pm

I have been messing around with phyto for a few years. I now have the cleanest culture of Tetraselmis I have ever had! I also would love to have a quick reference.
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Re: Phyto culturing.Why,Which and How.

Postby WDT » Mon Jun 07, 2010 9:24 am

Not sure if these are correct, I gathered this from google scholar searching for optimal conditions. Anyone care to comment on the importance of a light/dark cycle when growing certain types of phyto.

Isochrysis galbana
Salinity 25-35 ppt
Temp 24–26 °C

Tetraselmis
Salinity 25-35 ppt
Temp 19-21°C
"Variations in salinity and in nutrient concentration had a greater effect on the final biomass than on the growth velocity. The total protein of the culture and protein per cell increased when the salinity increased for a given nutrient concentration."

Pavlova
Salinity 30-35 ppt
Temp 20°C

Rhodomonas lens
Salinity 28-30 ppt
Temp 19-23°C

Nannochloropsis
Salinity 25-35 ppt
Temp 19-21°C

Thalassiosira weissflogii
Salinity 15-35 ppt
Temp 20°C
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Re: Phyto culturing.Why,Which and How.

Postby lokshin55 » Wed Nov 03, 2010 9:51 pm

I use 2L Erlenmeyer flasks. This is better than soda bottles because you can sterilize them (fill with boiling salt water). I buy them from Thermo Fisher, with caps. I dril a small hole in a cap and put in a 1 ml sterile pipette with sterile cotton plug. I also boil water. Before I started doing this, I could not propagate my phyto for more than couple months. Now it seems to be growing indefinitely.
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Re: Phyto culturing.Why,Which and How.

Postby Spikefin » Fri Nov 27, 2015 6:59 pm

Hey Luis I started another thread on this but should I go with the Tahitian iso over the other iso strains? What's special about this strain. I'm trying to culture parvo, a tonsa, and pseudo. I only want to culture one algae only and want something stable(as stable as possible anyway). I know about keeping stuff sterile and everything like that. Sanitation won't bother me. What about Tetra? Feel free to answer on either thread or both. Sorry bout asking twice just want your expertise on this.
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Re: Phyto culturing.Why,Which and How.

Postby chris melb » Sat Oct 07, 2017 12:00 am

Luis A M wrote: Stock cultures are kept pure and possibly sterile in flasks. Each flask is filled with 60 ml of medium, cotton stopped and sterilized in the microwave oven. When cool, 10 ml of a thriving culture is seeded with a sterile pipette.


Hi Luis, how long are you microwaving these 60ml stock cultures? Is it the full typical 10mins? I’d imagine this would bring them to boil if done separately.

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Re: Phyto culturing.Why,Which and How.

Postby Luis A M » Sun Oct 08, 2017 12:28 am

No,it is 3 min on,5 min rest.Then again 3=5 min.And endly 4 min on.A pyrex with a pint of water helps in preventing boiling. :wink:
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Re: Phyto culturing.Why,Which and How.

Postby chris melb » Sun Oct 08, 2017 10:32 am

Thanks Luis. I tried this and the culture boiled and overflowed out of the flasks. I’m using a 1000w microwave, perhaps I need to use more than pint of water in the Pyrex.
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Re: Phyto culturing.Why,Which and How.

Postby Luis A M » Tue Oct 10, 2017 10:27 am

Perhaps,try 1 liter :? I don't know how many watts my microwave delivers,I am in NYC right now.Are you using cotton stopped 100 ml ehrlenmeyers?.
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Re: Phyto culturing.Why,Which and How.

Postby chris melb » Tue Oct 10, 2017 4:41 pm

Hi Luis, yes I am. Last night I tried 1200ml of water in the Pyrex and had three 100ml ehrlenmeyers cotton topped each filled with 100ml of culture water and during the 2nd minute of the 4min stint they started to boil over. I assume it’s the length of radiation exposure (10mins in total) that is important here? Wondering how important the 4min block is and whether this can split into two shorter 2min blocks with a break in between.
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